Mechanism(s) of attenuation of Theileria annulata vaccine cell lines
نویسندگان
چکیده
منابع مشابه
Semi-quantitative Analysis of Expression of Various Genes in relation to Possible Markers for Theileria annulata Attenuation
The sporozoites of Theileria annulata invade bovine MHC II cells, where they differentiate into schizonts. The later can immortalize and induce fundamental changes in their host cells. Live attenuated vaccine is an important way of controlling T. annulata infection of cattle. Production is by prolonged cultivation of macroschizont-infected cells. The mechanisms underlying this transformation ...
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The purpose of this study was to examine the potential application of Sorbitol, Sucrose and Gelatin in combination with glycerol in order to Theileria annulata infected cell line cryo-preservation. The efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. As a result, the...
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BACKGROUND Bovine theileriosis results from infection with obligate intracellular protozoa of the genus Theileria. The phylogenetic relationships between two isolates of Theileria annulata, and 36 Theileria spp., as well as 6 outgroup including Babesia spp. and coccidian protozoa were analyzed using the 18S rRNA gene sequence. METHODS The target DNA segment was amplified by PCR. The PCR produ...
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The invasion of Theileria sporozoites into bovine leukocytes is rapidly followed by the destruction of the surrounding host cell membrane, allowing the parasite to establish its niche within the host cell cytoplasm. Theileria infection induces host cell transformation, characterised by increased host cell proliferation and invasiveness, and the activation of anti-apoptotic genes. This process i...
متن کاملPCR-based detection of Theileria infection and molecular characterization of Tams1 T. annulata vaccine strain
In order to develop a method for detecting and identification of Theileria annulata, the specific primers from the major merozoite-piroplasm surface antigen sequence of Theileria (Tams1) were used to detect the T. annulata by nested-PCR technique. The assay provides a valuable tool for the identification of Theileria annulata directly from clinical samples and enables determination of the infec...
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ژورنال
عنوان ژورنال: Tropical Medicine and International Health
سال: 1999
ISSN: 1360-2276,1365-3156
DOI: 10.1046/j.1365-3156.1999.00454.x